erythrocytes

Here, we report that a protein kinase-mediated signalling pathway involving host RBC PAK1 and MEK1, which do not have orthologues in the Plasmodium kinome, is selectively stimulated in Plasmodium falciparum-infected (versus uninfected) RBCs, as determined by the use of phospho-specific antibodies directed against the activated forms of these enzymes.

To examine the contamination by leukocytes of purified erythrocytes from human blood, 20 µl of whole blood was mixed with 10 ml of RPMI 1640, and the mixture was passed through a leukocyte isolation filter.

These new results on PfEMP1 antigen expression indicate that a re-evaluation of the molecular mechanisms involved in P. falciparum adhesion and of the accepted paradigm of absolutely mutually exclusive var gene transcription is required.

In this study we examined the downstream effects of reduced haemoglobin digestion on osmoprotection and nutrition. We found that inhibitors of haemoglobinases (plasmepsins, falcipains and aminopeptidases) did not cause premature haemolysis.

These observations prompt a modification of the current paradigms of the pathogenesis of malaria and clear the way to investigate the pathophysiology of P. vivax infections.

Rapid cell-mediated immune responses, characterized by production of proinflammatory cytokines, such as IFN-{gamma}, can inhibit intraerythrocytic replication of malaria parasites and thereby prevent onset of clinical malaria.

Rapid cell-mediated immune responses, characterized by production of proinflammatory cytokines, such as IFN-{gamma}, can inhibit intraerythrocytic replication of malaria parasites and thereby prevent onset of clinical malaria.

A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation columns was performed. The models applied in this study allow the prediction of capture efficiency if the initial infected cell concentration and the flow rate are known.

These findings demonstrate that the major PfEMP1 variant expressed by placental isolates exposes strain-transcendent epitopes that can be targeted by vaccination and may have application for pregnancy malaria vaccine development.

Invasion of host erythrocytes by Plasmodium falciparum merozoites requires attachment to the erythrocyte surface and the coordinated release of the contents of the apical organelles (micronemes and rhoptries) into the host cell.