A detailed protocol for membrane feeding is presented, as well as a detailed assessment of the precision of membrane feeding assays by allowing batches of mosquitoes to feedon the same gametocytaemic bloodmeal. When examining three batches of 50 mosquitoes that fed on aliquots of the same bloodmeal, we found a broad agreement in the assessment of infectiousness (i.e., infecting at least one mosquito), kappa 0.75, p<0.0001. On a continuous scale, we observed a strong association between mosquito infection rates in the different feeders (r≥0.88, p<0.0001). Our findings indicate that membrane feeding assays with the described protocol and 50 mosquito observations per experiment give an acceptable level of precision.
A seperate supplement to this article provides a detailed protocol for undertaking membrane feeding assays.
A video tutorial can be found on YouTube (click here).
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