Sri Lanka

We investigated possible variations that had not been reported previously, in the susceptibility to common insecticides and resistance mechanisms among the An. subpictus sibling species.

Emerged adults were identified using standard keys. DNA sequences of the D3 domain of 28S ribosomal DNA (rDNA) and the internal transcribed spacer-2 (ITS-2) of the morphologically identified An. annularis were determined.

We examined the frequency of Plasmodium falciparum chloroquine transporter (pfcrt; codons 72–76) haplotypes in Sri Lanka in 1996–1998 and 2004–2006 using a high-resolution melting assay.

Following progressive reduction in confirmed cases of malaria from 2002 to 2007 (41,411 cases in 2002, 10,510 cases in 2003, 3,720 cases in 2004, 1,640 cases in 2005, 591 cases in 2006, and 198 cases in 2007).

Diagnostic confusion may occur between dengue and malaria when febrile patients with thrombocytopenia return from travel to previous malaria endemic areas.

In comparison to the reference Sal-1 strain, among 402 world-wide isolates (302 global and 100 local), 121 aa polymorphisms and 138 haplotypes were recorded of which 3 aa polymorphisms and 21 haplotypes seem to be unique to Sri Lanka.

This study determines the use of nested PCR as a diagnostic tool to supplement field microscopy in symptomatic individuals suspected of being positive for malaria, and it explores its role in active case detection to identify asymptomatic parasite carriers.

The results suggest that An. subpictus species B/An. sundaicus breed in fresh, brackish and nearly saline water while An. subpictus species C and D do so in fresh and less brackish waters in Sri Lanka as in India.

Cytoadherence properties of two categories of Plasmodium falciparum isolates from Sri Lanka were assessed using CHO-CD36 and CHO-ICAM-1 stable transfected cell lines, and the association with the clinical disease in patients, from whom the parasites were obtained was also analyzed.

Here we investigated the level of genetic diversity in the PvMSP-142 gene fragment in 95 single clone P. vivax infections in Sri Lanka.