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anopheles coluzzii

Multimodal synergisms in host stimuli drive landing response in malaria mosquitoes

April 6, 2021 - 13:50 -- Open Access
Carnaghi M, Belmain SR, Hopkins RJ, Hawkes FM
Sci Rep. 2021 Apr 1;11(1):7379

Anopheles mosquitoes transmit malaria, which affects one-fifth of the world population. A comprehensive understanding of mosquito behaviour is essential for the development of novel tools for vector control and surveillance. Despite abundant research on mosquito behaviour, little is known on the stimuli that drive malaria vectors during the landing phase of host-seeking.

A PCR-RFLP method for genotyping of inversion 2Rc in Anopheles coluzzii

March 23, 2021 - 14:42 -- Open Access
Montanez-Gonzalez R, Vallera AC, Besansky NJ, et al.
Parasit Vectors. 2021 Mar 22;14(1):174

Genotyping of polymorphic chromosomal inversions in malaria vectors such as An. coluzzii Coetzee & Wilkerson is important, both because they cause cryptic population structure that can mislead vector analysis and control and because they influence epidemiologically relevant eco-phenotypes. The conventional cytogenetic method of genotyping is an impediment because it is labor intensive, requires specialized training, and can be applied only to one gender and developmental stage. Here, we circumvent these limitations by developing a simple and rapid molecular method of genotyping inversion 2Rc in An. coluzzii that is both economical and field-friendly. This inversion is strongly implicated in temporal and spatial adaptations to climatic and ecological variation, particularly aridity.

Chromosome-level genome assemblies of the malaria vectors Anopheles coluzzii and Anopheles arabiensis

March 17, 2021 - 16:50 -- Open Access
Zamyatin A, Avdeyev P, Liang J, Sharma A, Chen C, Lukyanchikova V, Alexeev N, Tu Z, Alekseyev MA, Sharakhov IV
Gigascience. 2021 Mar 15;10(3):giab017

Anopheles coluzzii and Anopheles arabiensis belong to the Anopheles gambiae complex and are among the major malaria vectors in sub-Saharan Africa. However, chromosome-level reference genome assemblies are still lacking for these medically important mosquito species.

High pyrethroid/DDT resistance in major malaria vector Anopheles coluzzii from Niger-Delta of Nigeria is probably driven by metabolic resistance mechanisms

March 17, 2021 - 09:34 -- Open Access
Muhammad A, Ibrahim SS, Mukhtar MM, Irving H, Abajue MC, Edith NMA, Da'u SS, Paine MJI, Wondji CS
PLoS One. 2021 Mar 11;16(3):e0247944

Entomological surveillance of local malaria vector populations is an important component of vector control and resistance management. In this study, the resistance profile and its possible mechanisms was characterised in a field population of the major malaria vector Anopheles coluzzii from Port Harcourt, the capital of Rivers state, in the Niger-Delta Region of Nigeria. Larvae collected in Port-Harcourt, were reared to adulthood and used for WHO bioassays. The population exhibited high resistance to permethrin, deltamethrin and DDT with mortalities of 6.7% ± 2.4, 37.5% ± 3.2 and 6.3% ± 4.1, respectively, but were fully susceptible to bendiocarb and malathion. Synergist bioassays with piperonylbutoxide (PBO) partially recovered susceptibility, with mortalities increasing to 53% ± 4, indicating probable role of CYP450s in permethrin resistance (χ2 = 29.48, P < 0.0001).

NOT Open Access | Potentiation Effects of Ficus sycomorus Active Fraction Against Permethrin-Resistant Field-Population of Anopheles coluzzii (Diptera: Culicidae)

March 9, 2021 - 15:39 -- NOT Open Access
Anosike CA, Babandi A, Ezeanyika LUS
Neotrop Entomol (2021)

Insecticide resistance in mosquitoes is increasing amidst growing cases of global malaria, leading to high fatality in mostly Africa. To overcome the resistance as well as environmental effects of the synthetic insecticides, preliminary insecticidal and botanical potentiating effects of sub-lethal concentration (LC25) Ficus sycomorus active fraction (AFFS) and its synergistic potential with standard insecticide permethrin were evaluated against malarial vector Anopheles coluzzii (Coetzee & Wilkerson) populations. The glutathione-S-transferase (GST) inhibitory activity of the AFFS was also investigated compared to standard GST inhibitor, diethyl meleate (DEM).

NOT Open Access | Novel genotyping approaches to easily detect genomic admixture between the major Afrotropical malaria vector species, Anopheles coluzzii and An. gambiae

February 16, 2021 - 15:44 -- NOT Open Access
Caputo B, Pichler V, Bottà G, De Marco C, Hubbart C, Perugini E, Pinto J, Rockett KA, Miles A, Della Torre A
Mol Ecol Resour. 2021 Feb 15

The two most efficient and most recently radiated Afrotropical vectors of human malaria - Anopheles coluzzii and An. gambiae - are identified by single-locus diagnostic PCR assays based on species-specific markers in a 4 Mb region on chromosome-X centromere. Inherently, these diagnostic assays cannot detect interspecific autosomal admixture shown to be extensive at the westernmost and easternmost extremes of the species range.

The Expression of Chemosensory Genes in Male Maxillary Palps of Anopheles coluzzii (Diptera: Culicidae) and An. quadriannulatus

February 15, 2021 - 15:09 -- Open Access
Athrey G, Popkin-Hall ZR, Takken W, Slotman MA
J Med Entomol. 2021 Feb 12:tjaa290

Because of its importance as a malaria vector, Anopheles coluzzii's Coetzee & Wilkerson olfactory system has been studied extensively. Among this work is a series of studies comparing the expression of chemosensory genes in olfactory organs in females and/or males of these species. These have identified species- and female-biased chemosensory gene expression patterns.

Plasmodium oocysts respond with dormancy to crowding and nutritional stress

February 8, 2021 - 10:41 -- Open Access
Habtewold T, Sharma AA, Wyer CAS, Masters EKG, Windbichler N, Christophides GK
Sci Rep. 2021 Feb 4;11(1):3090

Malaria parasites develop as oocysts in the mosquito for several days before they are able to infect a human host. During this time, mosquitoes take bloodmeals to replenish their nutrient and energy reserves needed for flight and reproduction. We hypothesized that these bloodmeals are critical for oocyst growth and that experimental infection protocols, typically involving a single bloodmeal at the time of infection, cause nutritional stress to the developing oocysts. Therefore, enumerating oocysts disregarding their growth and differentiation state may lead to erroneous conclusions about the efficacy of transmission blocking interventions.

Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus

January 26, 2021 - 15:32 -- Open Access
Grau-Bové X, Lucas E, Weetman D, et al.
PLoS Genet. 2021 Jan 21;17(1):e1009253

Vector population control using insecticides is a key element of current strategies to prevent malaria transmission in Africa. The introduction of effective insecticides, such as the organophosphate pirimiphos-methyl, is essential to overcome the recurrent emergence of resistance driven by the highly diverse Anopheles genomes. Here, we use a population genomic approach to investigate the basis of pirimiphos-methyl resistance in the major malaria vectors Anopheles gambiae and A. coluzzii. A combination of copy number variation and a single non-synonymous substitution in the acetylcholinesterase gene, Ace1, provides the key resistance diagnostic in an A. coluzzii population from Côte d'Ivoire that we used for sequence-based association mapping, with replication in other West African populations.

NOT Open Access | Mutagenesis of the orco odorant receptor co-receptor impairs olfactory function in the malaria vector Anopheles coluzzii

December 3, 2020 - 12:19 -- NOT Open Access
Sun H, Liu F, Ye Z, Baker A, Zwiebel LJ
Insect Biochem Mol Biol. 2020 Dec;127:103497

Mosquitoes rely heavily on their olfactory systems for host seeking, selection of oviposition sites, and avoiding predators and other environmental dangers. Of these behaviors, the preferential selection of a human blood-meal host drives the vectorial capacity of anthropophilic female Anopheles coluzzii mosquitoes. Olfactory receptor neurons (ORNs) are dispersed across several appendages on the head and express an obligate odorant receptor co-receptor (Orco) coupled with a "tuning" odorant receptor (OR) to form heteromeric, odor-gated ion channels in the membrane of these neurons.


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