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polymerase chain reaction

NOT Open Access | Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar

September 29, 2020 - 13:21 -- NOT Open Access
Win SY, Chel HM, Hmoon MM, Htun LL, Bawm S, Win MM, Murata S, Nonaka N, Nakao R, Katakura K
Acta Trop. 2020 Sep 22:105719

Village chicken production, a traditional, small-scale, and extensive backyard poultry industry, has been profitable for local farmers in Myanmar. However, there is scanty information available concerning the infection of these chickens with avian pathogens, including haemoprotozoan parasites. In the present study, we provide the first report of microscopic detection and molecular identification of Leucocytozoon and Plasmodium parasites from seven different areas of Myanmar. Leucocytozoon gametocytes were detected in 17.6% (81/461) of the blood smears from village chickens.

NOT Open Access | Congenital Malaria in Newborns Delivered to Mothers with Malaria-Infected Placenta in Blue Nile State, Sudan

August 3, 2020 - 15:43 -- NOT Open Access
Omer SA, Adam I, Noureldien A, Elhaj H, Guerrero-Latorre L, Silgado A, Sulleiro E, Molina I
J Trop Pediatr. 2020 Aug 1;66(4):428-434

Diagnosis of congenital malaria is complicated by the low density of the parasite circulating in the cord blood and/or the peripheral blood of the newborns. Molecular techniques are significantly more sensitive than blood smears in detecting low-level parasitemia. This study investigated the prevalence of congenital malaria by the use of the real-time polymerase chain reaction (real-time PCR) in 102 babies born to mothers with microscopically confirmed infected placenta from Blue Nile state, Sudan.

Summary of discordant results between rapid diagnosis tests, microscopy, and polymerase chain reaction for detecting Plasmodium mixed infection: a systematic review and meta-analysis

July 30, 2020 - 14:10 -- Open Access
Kotepui M, Kotepui KU, De Jesus Milanez G, Masangkay FR
Sci Rep. 2020 Jul 29; 10(1):12765

Malaria rapid diagnostic tests (RDTs) are widely used to detect malaria parasites among patients who suspected malaria infections in malaria-endemic areas where microscopy is unavailable. Nevertheless, little is known about the performance of RDTs in detecting Plasmodium mixed infections. The present study aimed to evaluate the discordant results between RDTs and microscopy/polymerase chain reaction (PCR) in detecting Plasmodium mixed infections.

Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivax

July 14, 2020 - 09:19 -- Open Access
Punitha Mahendran, Jonathan Wee Kent Liew, Amirah Amir, Xiao-Teng Ching and Yee-Ling Lau
Malaria Journal 2020 19:241, 10 July 2020

Plasmodium knowlesi and Plasmodium vivax are the predominant Plasmodium species that cause malaria in Malaysia and play a role in asymptomatic malaria disease transmission in Malaysia. The diagnostic tools available to diagnose malaria, such as microscopy and rapid diagnostic test (RDT), are less sensitive at detecting lower parasite density. Droplet digital polymerase chain reaction (ddPCR), which has been shown to have higher sensitivity at diagnosing malaria, allows direct quantification without the need for a standard curve. The aim of this study is to develop and use a duplex ddPCR assay for the detection of P. knowlesi and P. vivax, and compare this method to nested PCR and qPCR.

Therapeutic efficacy of artemether-lumefantrine in the treatment of uncomplicated Plasmodium falciparum malaria in Chewaka District, Ethiopia

July 14, 2020 - 09:17 -- Open Access
Abdulhakim Abamecha, Daniel Yilma, Wondimagegn Addisu, Hassan El-Abid, Achim Ibenthal, Harald Noedl, Delenasaw Yewhalaw, Mohieddine Moumni and Alemseged Abdissa
Malaria Journal 2020 19:240, 10 July 2020

The efficacy of artemether-lumefantrine (AL) for treatment of uncomplicated Plasmodium falciparum malaria in south-western Ethiopia is poorly documented. Regular monitoring of drug efficacy is an important tool for supporting national treatment policies and practice. This study investigated the therapeutic efficacy of AL for the treatment of P. falciparum malaria in Ethiopia.

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