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Pfcrt

Increase in the proportion of Plasmodium falciparum with kelch13 C580Y mutation and decline in pfcrt and pfmdr1 mutant alleles in Papua New Guinea

October 30, 2021 - 14:05 -- Open Access
Author(s): 
Naoko Yoshida, Masato Yamauchi, Ryosuke Morikawa, Francis Hombhanje and Toshihiro Mita
Reference: 
Malaria Journal 2021 20:410, 19 October 2021

The C580Y mutation in the Plasmodium falciparum kelch13 gene is the most commonly observed variant in artemisinin-resistant isolates in the Greater Mekong Subregion (GMS). Until 2017, it had not been identified outside the GMS, except for Guyana/Amazonia. In 2017, three parasites carrying the C580Y mutation were identified in Papua New Guinea (PNG). As the C580Y allele rapidly spread in the GMS, there is concern that this mutant is now spreading in PNG.

NOT Open Access | Mutations in Pfcrt and Pfmdr1 genes of Plasmodium falciparum isolates from two sites in Northcentral and Southwest Nigeria

August 25, 2021 - 16:56 -- NOT Open Access
Author(s): 
Agomo CO, Mishra N, Olukosi YA, Gupta R, Kamlesh K, Aina OO, Awolola ST
Reference: 
Infect Genet Evol. 2021 Aug 19:105042

The ability of malaria parasites to develop resistance to antimalarial drugs has made it necessary to continuously survey malaria parasite populations for resistance markers. Mutations in specific malaria parasite genes confer resistance to antimalarial drugs. The study compared mutations in Pfcrt and Pfmdr1 genes of P. falciparum from two ecologically different areas of Nigeria. Plasmodium falciparum dried blood spots collected from New Bussa (Northcentral Nigeria) and Ijede (Southwest Nigeria) were analysed by PCR-RFLP for Pfcrt, K76 T, Pfmdr1, N86Y and Y184F mutations.

NOT Open Access | Epitope-specific IgG pools identify PfCRT monomer and homodimer polypeptides that are differentially phosphorylated at Ser(411) in Plasmodium falciparum

June 15, 2021 - 14:45 -- NOT Open Access
Author(s): 
Baakdah F, Georges E
Reference: 
Biochem Biophys Res Commun. 2021 Jun 11;557:261-266

The Plasmodium falciparum chloroquine resistance transporter (PfCRT) is a phospho-protein with three identified phosphorylation sites (Ser33, Ser411 and Thr416) at its cytosolic N- and C-termini. In this study, we report on the characterization of PfCRT anti-serum and show the presence of three epitope-specific immunoglobulin (IgG) pools (i.e., IgG-P1, P2, and P3), each recognizing a different epitope in PfCRT cytoplasmic C-terminal.

Molecular surveillance of anti-malarial resistance pfcrt, pfmdr1, and pfk13 polymorphisms in African Plasmodium falciparum imported parasites to Wuhan, China

May 6, 2021 - 07:19 -- Open Access
Author(s): 
Weijia Cheng, Xiaonan Song, Huabing Tan, Kai Wu and Jian Li
Reference: 
Malaria Journal 2021 20:209, 1 May 2021

Imported malaria parasites with anti-malarial drug resistance (ADR) from Africa is a serious public health challenge in non-malarial regions, including Wuhan, China. It is crucial to assess the ADR status in African Plasmodium falciparum isolates from imported malaria cases, as this will provide valuable information for rational medication and malaria control.

NOT Open Access | Plasmodium falciparum Isolates Carrying pfk13 Polymorphisms Harbor the SVMNT Allele of pfcrt in Northwestern Indonesia

July 27, 2020 - 12:26 -- NOT Open Access
Author(s): 
Lubis IND, Wijaya H, Lubis M, Lubis CP, Beshir KB, Sutherland CJ
Reference: 
Antimicrob Agents Chemother. 2020 Jul 22; 64(8):e02539-19

Artemisinin-based combination therapy (ACT) is the first-line antimalarial regimen in Indonesia. Susceptibility of Plasmodium falciparum to artemisinin is falling in the Greater Mekong subregion, but it is not known whether the efficacy of current combinations is also threatened in nearby Sumatera. We evaluated the genetic loci pfcrt, pfmdr1, and pfk13, considered to be under selection by artemisinin combination therapy, among 404 P. falciparum infections identified by PCR detection in a cross-sectional survey of 3,731 residents of three regencies.

Polymorphisms of pfcrt, pfmdr1, and K13-propeller genes in imported falciparum malaria isolates from Africa in Guizhou province, China

July 20, 2020 - 15:28 -- Open Access
Author(s): 
She D, Wang Z, Liang Q, Lu L, Huang Y, Zhang K, An D, Wu J
Reference: 
BMC Infect Dis. 2020 Jul 16;20(1):513

Imported falciparum malaria from Africa has become a key public health challenge in Guizhou Province since 2012. Understanding the polymorphisms of molecular markers of drug resistance can guide selection of antimalarial drugs for the treatment of malaria. This study was aimed to analyze the polymorphisms of pfcrt, pfmdr1, and K13-propeller among imported falciparum malaria cases in Guizhou Province, China.

Prevalence of mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, and association with ex vivo susceptibility to common anti-malarial drugs against African Plasmodium falciparum isolates

June 8, 2020 - 15:27 -- Open Access
Author(s): 
Francis Tsombeng Foguim, Hervé Bogreau, Mathieu Gendrot, Joel Mosnier, Isabelle Fonta, Nicolas Benoit, Rémy Amalvict, Marylin Madamet, Sharon Wein and Bruno Pradines
Reference: 
Malaria Journal 2020 19:201, 5 June 2020

The Plasmodium falciparum chloroquine transporter gene (pfcrt) is known to be involved in chloroquine and amodiaquine resistance, and more particularly the mutations on the loci 72 to 76 localized within the second exon. Additionally, new mutations (T93S, H97Y, C101F, F145I, M343L, C350R and G353V) were recently shown to be associated with in vitro reduced susceptibility to piperaquine in Asian or South American P. falciparum strains. However, very few data are available on the prevalence of these mutations and their effect on parasite susceptibility to anti-malarial drugs, and more particularly piperaquine in Africa.

Not Open Access | Plasmodium faciparum carrying pfk13 polymorphisms harbour the SVMNT allele of pfcrt in north-western Indonesia

May 13, 2020 - 14:38 -- NOT Open Access
Author(s): 
Lubis IND, Wijaya H, Lubis M, Lubis CP, Beshir KB, Sutherland CJ
Reference: 
Antimicrob Agents Chemother. 2020 May 11. pii: AAC.02539-19

Artemisinin-based combination therapy is the first-line antimalarial regimen in Indonesia. Susceptibility of Plasmodium falciparum to artemisinin is falling in the Greater Mekong sub-Region, but it is not known whether the efficacy of current combinations is also threatened in nearby Sumatera. We evaluated the genetic loci pfcrt, pfmdr1 and pfk13, considered to be under selection by artemisinin combination therapy, among 404 P. falciparum infections identified by PCR detection in a cross-sectional survey of 3,731 residents of three Regencies.

NOT Open Access | Turning the tide: targeting PfCRT to combat drug-resistant P. falciparum

May 7, 2020 - 13:28 -- NOT Open Access
Author(s): 
Small-Saunders JL, Hagenah LM, Fidock DA
Reference: 
Nat Rev Microbiol. 2020 May;18(5):261-262

Resistance to the current first-line antimalarials threatens the control of malaria caused by the protozoan parasite Plasmodium falciparum and underscores the urgent need for new drugs with novel modes of action.

Molecular surveillance of Pfcrt and k13 propeller polymorphisms of imported Plasmodium falciparum cases to Zhejiang Province, China between 2016 and 2018

February 10, 2020 - 16:08 -- Open Access
Author(s): 
Xiaoxiao Wang, Wei Ruan, Shuisen Zhou, Fang Huang, Qiaoyi Lu, Xinyu Feng and He Yan
Reference: 
Malaria Journal 2020 19:59, 4 February 2020

Resistance to anti-malarial drugs hinders malaria elimination. Monitoring the molecular markers of drug resistance helps improve malaria treatment policies. This study aimed to assess the distribution of molecular markers of imported Plasmodium falciparum infections.

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