The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 11110 malaria professionals are enjoying the free benefits of MalariaWorld today

PhD thesis: Development and demise of Plasmodium liver stage parasites - The hunt for a Genetically Attenuated Malaria Vaccine

July 3, 2013 - 14:19 -- Bart G.J. Knols
Ivo Ploemen
MWJ 2013, 4, 13
Article type: 

A considerable effort is currently underway to develop a malaria vaccine based on live Plasmodium falciparum sporozoites. The first requisite of a sporozoite vaccine is the guarantee of parasite arrest prior to the onset of the pathogenic blood stage. Immunisation with genetically attenuated parasites (GAP) that arrest in the liver forms a promising approach. Work in this thesis describes the development and characterisation of a P. berghei Δb9Δslarp GAP that fully arrests in the liver. Immunisation of multiple mouse strains with low numbers of Δb9Δslarp GAP resulted in sterile protection. The Δb9Δslarp GAP is there- fore the leading GAP vaccine candidate. Work in this the- sis further describes the effect of varying the parameters of sporozoite inoculation on parasite liver load. These findings provide a rationale for the design of clinical trials aimed at the administration of live attenuated P. falciparum sporozoites.

[Note: Full copy of PhD thesis attached]

PDF icon MWJ2013_4_13.pdf375.1 KB
PDF icon PhD thesis_Ploemen _2013.pdf16.15 MB